Insertion junction: LMJ.RY0402.195411_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.195411
Chromosome:chromosome 7
Location:6150155
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g355950 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGACGGCAAGGTTAGAGGGCGGTCGAGGA
Internal bar code:CTGGCTACCCATGGGGGCTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:30
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGGTGTATTGGGTGCTG
Suggested primer 2:CAACACCACGCTTTCCCTAT