| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195413 |
| Chromosome: | chromosome 3 |
| Location: | 9043394 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g210513 | (1 of 2) K05894 - 12-oxophytodienoic acid reductase (OPR) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCACCCACGCTGCAGCCCTAGGCACAC |
| Internal bar code: | GCCCGGGCTGGTATATCACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 99.7619 |
| LEAP-Seq n confirming: | 419 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTACACCTGGTAGCCCT |
| Suggested primer 2: | GAAAGCTTCAACAAGCAGGG |