| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.195421 |
| Chromosome: | chromosome 16 |
| Location: | 3075677 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665328 | 3'UTR/CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGGCCCTACGCCGCCGGCAGGTCCTGCT |
| Internal bar code: | GTCAGCCTGAAGACACCTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 99.3506 |
| LEAP-Seq n confirming: | 153 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTACACGACAAGGGGACA |
| Suggested primer 2: | CAGTAGGCAGATGCTGTGGA |