| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.195435 |
| Chromosome: | chromosome 16 |
| Location: | 4492300 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g667451 | TLP3,CAV6 | Tubby-like protein; (1 of 5) PTHR10037//PTHR10037:SF62 - VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM // SODIUM CHANNEL PROTEIN PARA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCTAGCGTGCTCGATGGATACGCTCTC |
| Internal bar code: | ACGTCGAAGCCCGGCCTCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 99.1868 |
| LEAP-Seq n confirming: | 4147 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGGAGTACGACGGCATGA |
| Suggested primer 2: | GAAGGAAGCCTCAGTTGTCG |