Insertion junction: LMJ.RY0402.195436_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.195436
Chromosome:chromosome 9
Location:535440
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g403950 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATGCTGGTCGGTCGTCGCAGGCATGTCG
Internal bar code:CCGCGGATCCGTATTCGACGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:551
LEAP-Seq percent confirming:99.5017
LEAP-Seq n confirming:599
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCGTTGTGGAAGGTGGAC
Suggested primer 2:TTAACGTCAGGGGTCTACCG