Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.195436 |
Chromosome: | chromosome 9 |
Location: | 535440 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g403950 | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATGCTGGTCGGTCGTCGCAGGCATGTCG |
Internal bar code: | CCGCGGATCCGTATTCGACGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 551 |
LEAP-Seq percent confirming: | 99.5017 |
LEAP-Seq n confirming: | 599 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTCGTTGTGGAAGGTGGAC |
Suggested primer 2: | TTAACGTCAGGGGTCTACCG |