| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.195450 |
| Chromosome: | chromosome_6 |
| Location: | 7211004 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g297850 | DHC12 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAACTGTAAAACGAACGCCGAGGTTTCGTT |
| Internal bar code: | GTCTATGATGGATTCCCAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 98.6652 |
| LEAP-Seq n confirming: | 887 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCCCAAGTGGTGAAGTG |
| Suggested primer 2: | CACGTACACCAAGCTGATGC |