Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.195457 |
Chromosome: | chromosome_12 |
Location: | 8686790 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g546400 | DLR2,DLC7b,DLC7B | Flagellar inner arm I1/f axonemal dynein, LC7b | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCGTTGTCCTGACCCCTGCTATGTCTCGGC |
Internal bar code: | TTTTGGGCTTTATTAGCTATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1178 |
LEAP-Seq percent confirming: | 99.5208 |
LEAP-Seq n confirming: | 623 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATACCCAAGCGGTGACAGAC |
Suggested primer 2: | TTCCATCGATTCATCGTCAA |