| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.195469 |
| Chromosome: | chromosome 8 |
| Location: | 2304075 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370350 | (1 of 6) PF09409 - PUB domain (PUB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCCCAACGGTCAACGCTGACCCCCATG |
| Internal bar code: | GAGCCACCGACCTTACCCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 121 |
| LEAP-Seq percent confirming: | 99.4741 |
| LEAP-Seq n confirming: | 1135 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGACAAGTTGCGAGATG |
| Suggested primer 2: | GTCCGTCAGTGCTCAACTCA |