Insertion junction: LMJ.RY0402.195469_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.195469
Chromosome:chromosome 8
Location:2304075
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g370350 (1 of 6) PF09409 - PUB domain (PUB) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTCCCAACGGTCAACGCTGACCCCCATG
Internal bar code:GAGCCACCGACCTTACCCCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:121
LEAP-Seq percent confirming:99.4741
LEAP-Seq n confirming:1135
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCGACAAGTTGCGAGATG
Suggested primer 2:GTCCGTCAGTGCTCAACTCA