Insertion junction: LMJ.RY0402.195496_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.195496
Chromosome:chromosome 14
Location:792236
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g613050 (1 of 80) IPR003882 - Pistil-specific extensin-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACTGACCCAAACACACACACAAACAC
Internal bar code:AGGTACGGGCCATCCAGTTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:745
LEAP-Seq percent confirming:79.7998
LEAP-Seq n confirming:2232
LEAP-Seq n nonconfirming:565
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCTAATCAGGCCTCTTGCAG
Suggested primer 2:CCCTAAAACGTCACATGGCT