Insertion junction: LMJ.RY0402.195519_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.195519
Chromosome:chromosome 11
Location:2697757
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g476850 PF2,DRC4,GAS8 (1 of 1) PTHR31543//PTHR31543:SF0 - FAMILY NOT NAMED // GROWTH ARREST-SPECIFIC PROTEIN 8; Nexin-dynein regulatory complex 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGAACACCCTCGCACACTCCTCCTGGTG
Internal bar code:TGGCAGTATCAGCCGGGGTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:598
LEAP-Seq percent confirming:99.8213
LEAP-Seq n confirming:2234
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTTCACTTCTGGCCTTGC
Suggested primer 2:CTATGCAACAAGCGATCGAA