| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.195551 |
| Chromosome: | chromosome_7 |
| Location: | 3591148 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g336650 | MLH3 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCGCCACCGCAGCTCGCAGACCCCCCACG |
| Internal bar code: | TTAACCTTTTGGCTGTAACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 157 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTACCTTACATCACCCGC |
| Suggested primer 2: | CGGCTTCTAGGAATGCTTTG |