Insertion junction: LMJ.RY0402.195559_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.195559
Chromosome:chromosome 7
Location:256292
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g313950 (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGGACGAGGGGACCGTGGCGGGTTTCC
Internal bar code:GAAGGCAAGCATGAGACAGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1032
LEAP-Seq percent confirming:98.6048
LEAP-Seq n confirming:2615
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:TGAAGACGCGATTACGACAG
Suggested primer 2:GAACCACAGAAAGGTGCGAT