Insertion junction: LMJ.RY0402.195586_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.195586
Chromosome:chromosome 7
Location:5420505
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g350400 PIN3 Peptidyl-prolyl cis-trans isomerase, parvulin-type; (1 of 1) 2.7.7.80//2.8.1.11//5.2.1.8 - Molybdopterin-synthase adenylyltransferase // Molybdopterin-synthase sulfurtransferase / Molybdopterin synthase sulfurylase // Peptidylprolyl isomerase / Rotamase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACCGTACAGGCGGTCGGTCGGTATCTTG
Internal bar code:GCGTAAGGACGGTAGGGGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:760
LEAP-Seq percent confirming:99.4599
LEAP-Seq n confirming:2578
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ATACCCATGTGACGAACGGT
Suggested primer 2:TCGCCTGAGTCAGCACATAC