| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.195598 |
| Chromosome: | chromosome 3 |
| Location: | 3417464 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167000 | CrUBC3,CreSCE1b,CrUbcE2_2,SCE2,UBC3 | (1 of 3) K10577 - ubiquitin-conjugating enzyme E2 I (UBE2I, UBC9); SUMO-conjugating enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCCTTGTTCATCACGCCCACTTTGTCC |
| Internal bar code: | TGCGCCCCCGCCTACATTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 988 |
| LEAP-Seq percent confirming: | 99.2647 |
| LEAP-Seq n confirming: | 4995 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGACCTATGCAGGTGTTG |
| Suggested primer 2: | AGGTATGCATGCCGGTAAAC |