| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.195643 |
| Chromosome: | chromosome 17 |
| Location: | 3732245 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727400 | HRP8 | Hydroxyproline-rich glycoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGCCATGCGCACCTGCTCATCATACAG |
| Internal bar code: | CAAAGAAGATTAGGCACCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 126 |
| LEAP-Seq percent confirming: | 98.4375 |
| LEAP-Seq n confirming: | 252 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCATGTCACAGTTTTGC |
| Suggested primer 2: | ATTTTCACAGCCCCTGACAC |