| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.195650 |
| Chromosome: | chromosome 7 |
| Location: | 5479232 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350867 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGTTTGCCTCGAAACGCGGTTTGAAAC |
| Internal bar code: | GTGGAGCTAGACAGTGCGATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 810 |
| LEAP-Seq percent confirming: | 98.7772 |
| LEAP-Seq n confirming: | 2908 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGCTACTGGAAAAGCCA |
| Suggested primer 2: | TATCCATCGGAGTACGAGCC |