| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.195653 |
| Chromosome: | chromosome 9 |
| Location: | 6609480 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408464 | (1 of 1) PF10637 - Oxoglutarate and iron-dependent oxygenase degradation C-term (Ofd1_CTDD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGGGATGGTGGGTTGGGGTTGGGGTTT |
| Internal bar code: | ATCACGGTAGTGTTCTTAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 99.2701 |
| LEAP-Seq n confirming: | 1224 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTTGACGAAGCGCATGA |
| Suggested primer 2: | GGTTTGCCTACCGTCTACCA |