| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.195715 |
| Chromosome: | chromosome 6 |
| Location: | 8212540 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305600 | ADH9 | Alcohol dehydrogenase; (1 of 1) 1.1.1.329 - 2-deoxy-scyllo-inosamine dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACAGCCAATGCTGCAGCCCAGTGAGTC |
| Internal bar code: | GTTAAGCGATGGGCCCCAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 99.7846 |
| LEAP-Seq n confirming: | 2779 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAAGGGAACTCTCAAGG |
| Suggested primer 2: | TGATGACCTCGCACTCTCAC |