| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.195728 |
| Chromosome: | chromosome 1 |
| Location: | 1016498 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005813 | POB31 | Proteome of basal body 31 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCTGCTCTTCACGCACCGCGCCCCGGT |
| Internal bar code: | AGCTGGAGCAGTAGTTGCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1065 |
| LEAP-Seq percent confirming: | 99.6575 |
| LEAP-Seq n confirming: | 2910 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACATTTTGACCTGGGAGT |
| Suggested primer 2: | AATTCTCGCGCTACCTGAAA |