Insertion junction: LMJ.RY0402.195729_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.195729
Chromosome:chromosome 15
Location:981432
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g639100 FAP195 Flagellar Associated Protein 195 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGATTGGGGCGTGGGATTGGGGCCCAAG
Internal bar code:CTTGGAGTCACTTCTCAGGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:571
LEAP-Seq percent confirming:99.5816
LEAP-Seq n confirming:1190
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCCATACCACAGTTTTCGCT
Suggested primer 2:AAGGAGTGAAGGCAATGTGG