| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.195729 |
| Chromosome: | chromosome 15 |
| Location: | 981436 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639100 | FAP195 | Flagellar Associated Protein 195 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACCGTGCCTGTGCCCGCACCCCGCCCG |
| Internal bar code: | CGGCCAATACAGTATTTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 99.7207 |
| LEAP-Seq n confirming: | 714 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATACCACAGTTTTCGCT |
| Suggested primer 2: | AAGGAGTGAAGGCAATGTGG |