| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195767 |
| Chromosome: | chromosome 3 |
| Location: | 7743727 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203451 | CYA5 | Adenylate cyclase; (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATAGATGCAGCAGGTGCCGAGCGCTGC |
| Internal bar code: | CCACACAGGGGACGGGGAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 928 |
| LEAP-Seq percent confirming: | 98.4923 |
| LEAP-Seq n confirming: | 22081 |
| LEAP-Seq n nonconfirming: | 338 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGTGTTGATGATGATGC |
| Suggested primer 2: | TATGCATGACTAACGCTCGC |