Insertion junction: LMJ.RY0402.195768_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.195768
Chromosome:chromosome 3
Location:4390000
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g175500 (1 of 2) K05770 - benzodiazapine receptor (BZRP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGTCAGTCGTGGACTCATCGTGCGCCTT
Internal bar code:CGAGCAATGCGCGGGTGTTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:663
LEAP-Seq percent confirming:99.7375
LEAP-Seq n confirming:380
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGACGAACCTACAGACCCAT
Suggested primer 2:ACTTACCCCTTTCCGTTGCT