| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195824 |
| Chromosome: | chromosome 17 |
| Location: | 2108040 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712300 | (1 of 5) PF04232 - Stage V sporulation protein S (SpoVS) (SpoVS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTGTTCCTGGATTCCTGGCAGCAGGCC |
| Internal bar code: | CAGATGCCGGGCCAATGCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 283 |
| LEAP-Seq percent confirming: | 99.4545 |
| LEAP-Seq n confirming: | 547 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCCACACCAACACAACG |
| Suggested primer 2: | CTAGCACTCTGCGCATCAAG |