| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195840 |
| Chromosome: | chromosome 6 |
| Location: | 6298729 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g291500 | RWP6 | (1 of 16) PF02042 - RWP-RK domain (RWP-RK); RWP-RK transcription factor | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTACCTGTTTGTTTGACTGAGCCGATCC |
| Internal bar code: | ATCGACACGTGCAGGTTCGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 99.7497 |
| LEAP-Seq n confirming: | 5181 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACAAGCTAGGGTGAGGC |
| Suggested primer 2: | GCGGATTATAAGAATGCGGA |