| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.195853 |
| Chromosome: | chromosome 3 |
| Location: | 9176914 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g212753 | (1 of 1) PTHR21496//PTHR21496:SF6 - FERREDOXIN-RELATED // 3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE-LIKE PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCAGAACCCGAGCAGCAGGGGGAGACA |
| Internal bar code: | GCTCAGAAGGTAGTTCTGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 665 |
| LEAP-Seq percent confirming: | 99.4123 |
| LEAP-Seq n confirming: | 2030 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCACCCTAGCCAAACTCT |
| Suggested primer 2: | GGACTCCTGAACTTCCCACA |