| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195881 |
| Chromosome: | chromosome 9 |
| Location: | 4402315 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395436 | subunit 4 of splicing factor 3b (SF3b); (1 of 1) PTHR24012//PTHR24012:SF370 - FAMILY NOT NAMED // RNA-BINDING PROTEIN 44 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCCGCCAGGCGGGCCGGCTGCTGGCTC |
| Internal bar code: | TCTGGTCGCCCGATGATGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 99.8433 |
| LEAP-Seq n confirming: | 637 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGTCTTGTCTCTAGGCG |
| Suggested primer 2: | AGCCAAATAAGCGCAAGCTA |