Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.195901 |
Chromosome: | chromosome_1 |
Location: | 1824660 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g009800 | FAP289 | Flagellar Associated Protein, coiled-coil | antisense | 5'UTR_intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCTCATAACGGGTTTGCATTGCCGAAGTT |
Internal bar code: | GCGCTCATGATCACGTTAGAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 353 |
LEAP-Seq percent confirming: | 99.6122 |
LEAP-Seq n confirming: | 4881 |
LEAP-Seq n nonconfirming: | 19 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACCGGGAGAAGGAAGGTTA |
Suggested primer 2: | GGACAACTCTTCTTCCAGCG |