| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.195914 |
| Chromosome: | chromosome 3 |
| Location: | 3351906 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166600 | DIV147 | (1 of 1) PTHR13366//PTHR13366:SF0 - MALARIA ANTIGEN-RELATED // HEAT REPEAT-CONTAINING PROTEIN 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTTTGTATTCCCTCAAATGCCTCCCAT |
| Internal bar code: | CTGACCAGCGGGAGCGTGATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 97.9943 |
| LEAP-Seq n confirming: | 684 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGATGAGCTGTGTAGACGA |
| Suggested primer 2: | GACAAGGCTGACCTTCTTGC |