| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.195935 |
| Chromosome: | chromosome 1 |
| Location: | 5967958 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042450 | (1 of 14) IPR006626//IPR011050 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGAATATGAATTCCCTCAGGAAGAGGC |
| Internal bar code: | AATCGACGGAAATGTTGCCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 99.657 |
| LEAP-Seq n confirming: | 3196 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCCTTCTTGTTTAGGGGT |
| Suggested primer 2: | GCAAATGTATGCCATTCACG |