| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195956 |
| Chromosome: | chromosome 2 |
| Location: | 7239190 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g145800 | MDN3,MDH3 | NAD-dependent malate dehydrogenase 3; (1 of 1) K00025 - malate dehydrogenase (MDH1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGAGGAGGGCAGCGAGGGCAAGGCGGG |
| Internal bar code: | CATCCTGAGTGTAAAGTTAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 924 |
| LEAP-Seq percent confirming: | 99.5003 |
| LEAP-Seq n confirming: | 3186 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCATTACCTCCCAGGCTC |
| Suggested primer 2: | ACACACTTGAGGAGTTGGGG |