| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.195962 |
| Chromosome: | chromosome_8 |
| Location: | 3497231 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g375050 | FAP86 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACTTGTGGGAGTCCTTTTATGCGCTAAGAT |
| Internal bar code: | GTGCGTTGCGGCGACGGCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 99.8573 |
| LEAP-Seq n confirming: | 700 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACCCGTCCCAGCAACTA |
| Suggested primer 2: | GCGGGAGATGACAATCAAAT |