| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.195975 |
| Chromosome: | chromosome 9 |
| Location: | 4052048 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393284 | RLS8 | RegA/Rls-like protein; (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATATCAAGATAATAGTCCTTCATCGCGG |
| Internal bar code: | GGGCGATCAGTAGTAGGTGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 96.0765 |
| LEAP-Seq n confirming: | 1910 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTGGTACGTTCGGGTCT |
| Suggested primer 2: | TGGCGATGCATGTAGAGAAG |