| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.195977 |
| Chromosome: | scaffold 18 |
| Location: | 220563 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749797 | FAP281 | Flagellar Associated Coiled-Coil Protein 281 | 3'UTR_intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCAGGCCGGGGCGCACCACCTGGTGGCA |
| Internal bar code: | CCTTCCACACAGGCTCCCCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 99.8175 |
| LEAP-Seq n confirming: | 3281 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACCCGCAACTGTAACCA |
| Suggested primer 2: | TAAAGTCCCGTAGGGTGCAG |