| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196029 |
| Chromosome: | chromosome 7 |
| Location: | 1268526 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321700 | ADH3 | Alcohol dehydrogenase; (1 of 1) PTHR11695//PTHR11695:SF281 - ALCOHOL DEHYDROGENASE RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCAGCTCGTGCGTGATGATCCTGAAGC |
| Internal bar code: | ATGAGGTGACTAGTGGTGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 99.517 |
| LEAP-Seq n confirming: | 6593 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCTGCTACAATACGCAA |
| Suggested primer 2: | AGTGGCGCATACCTAACGAC |