| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.196060 |
| Chromosome: | chromosome 8 |
| Location: | 4042412 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379300 | (1 of 1) PTHR12459//PTHR12459:SF6 - UNCHARACTERIZED // TRANSMEMBRANE PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACGCGGCTGCAATTCTCCTTGCAGTGC |
| Internal bar code: | TAATGTCTGCAGTTTCCTATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGTGACAGTGTGGCTT |
| Suggested primer 2: | CAGGAGGCGGTCAGTAAGAG |