| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.196083 |
| Chromosome: | chromosome 16 |
| Location: | 5107631 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683953 | (1 of 19) PTHR31251//PTHR31251:SF1 - FAMILY NOT NAMED // SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 10-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTGTAGTTGCTGCAGTAGTGCCGGGGG |
| Internal bar code: | GCACTCTATTCGGTTCGCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 227 |
| LEAP-Seq percent confirming: | 99.1758 |
| LEAP-Seq n confirming: | 361 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCAGTGTCAATAAGGC |
| Suggested primer 2: | ACACCAAGGAACAATCCAGC |