| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.196132 |
| Chromosome: | chromosome 11 |
| Location: | 1199119 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467692 | (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACGTGTGAGTCACCATCATTCCCATGC |
| Internal bar code: | CTTGTAGCTTGCAGGGGTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 32.9426 |
| LEAP-Seq n confirming: | 815 |
| LEAP-Seq n nonconfirming: | 1659 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTCTGTACTTGCTCCTG |
| Suggested primer 2: | CGTGTGTGTGAGTGTAGGGG |