Insertion junction: LMJ.RY0402.196174_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.196174
Chromosome:chromosome_10
Location:1638055
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre10.g429750 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GTGCTAGACAATCCCCAGGTGCGTGCCAGT
Internal bar code:TTATCGTTATCCCTTCGTCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1118
LEAP-Seq percent confirming:95.3125
LEAP-Seq n confirming:122
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGGCAATATGCTTCATGG
Suggested primer 2:GCACGTGTTAGTCCCCAACT