Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.196180 |
Chromosome: | chromosome 14 |
Location: | 2427573 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g624650 | (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCCAAACCCCGCCTACCTGGGATCCAG |
Internal bar code: | GTTGGACGTTGACGAAGACAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 443 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 564 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AATTGGACGATGGACGCTAC |
Suggested primer 2: | GAGCAAGGAAAGCAATACGC |