Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.196198 |
Chromosome: | chromosome 2 |
Location: | 1862098 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g087450 | (1 of 4) PTHR16255:SF4 - SPORULATION PROTEIN RMD8 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACATGAGCCCCTTGCATTTGCGGGAGC |
Internal bar code: | GCCCAGGAGACTTTCCGCGTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 956 |
LEAP-Seq percent confirming: | 99.3604 |
LEAP-Seq n confirming: | 9631 |
LEAP-Seq n nonconfirming: | 62 |
LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCATGCCAAGATAGTGCAGA |
Suggested primer 2: | CTTGAGGTCCTACCCAACCA |