Insertion junction: LMJ.RY0402.196205_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.196205
Chromosome:chromosome 7
Location:2212720
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g327200 (1 of 3) PF00534//PF13579 - Glycosyl transferases group 1 (Glycos_transf_1) // Glycosyl transferase 4-like domain (Glyco_trans_4_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAGGACGACCTGGGCGCATACCGCATCG
Internal bar code:GGGCCCGTACGTATGTCGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:265
LEAP-Seq percent confirming:99.5575
LEAP-Seq n confirming:225
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCTAGCTGGATGGCTAATGC
Suggested primer 2:AAGGTTAGATTGCCCGGTTT