| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196220 |
| Chromosome: | chromosome 1 |
| Location: | 7981045 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g068742 | (1 of 4) PF01424 - R3H domain (R3H) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGGAGGGGGCGTGTCATCCCTCTAGGC |
| Internal bar code: | CCGGACTCGTGTGCTTGCACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 352 |
| LEAP-Seq percent confirming: | 99.7573 |
| LEAP-Seq n confirming: | 4933 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGGTAATGCGTGTATTG |
| Suggested primer 2: | CCCTACAGCTTCTGTCAGCC |