| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196227 |
| Chromosome: | chromosome 9 |
| Location: | 7621196 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414850 | (1 of 2) 5.4.99.9 - UDP-galactopyranose mutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTAAGTGTGCAACACAGCAGTGTACAGC |
| Internal bar code: | GTGGTCAGATCTCTCGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 131 |
| LEAP-Seq percent confirming: | 78.4314 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTGCATGTATGTGGACC |
| Suggested primer 2: | CGCGTACTGATCACTTGCAT |