Insertion junction: LMJ.RY0402.196228_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.196228
Chromosome:chromosome 8
Location:4449538
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g382250 (1 of 4) PTHR11584//PTHR11584:SF377 - SERINE/THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCGCAGGCGTGCCGGTAAGACAGTATG
Internal bar code:ACCACGCACTGCGACATGTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:517
LEAP-Seq percent confirming:84.6154
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGATGGCCACCTACCTGT
Suggested primer 2:TCCGGTTTGAGCCTTTATTG