Insertion junction: LMJ.RY0402.196231_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.196231
Chromosome:chromosome 10
Location:5320347
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g457750 SULTR1 (1 of 2) K18059 - sulfate transporter 4 (SULTR4); Sulfate transporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGTGAGCGGCAGGCAGACAATGTCGGTG
Internal bar code:CCGGCAGGGGGACGCTGCTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1013
LEAP-Seq percent confirming:99.8679
LEAP-Seq n confirming:3025
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTAGTTGTGTCGTGGTTG
Suggested primer 2:ACCATGAAGGAGGTGACCAG