Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.196262 |
Chromosome: | chromosome_17 |
Location: | 3321753 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g723300 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTAGCGCAGGCGCGGGCTCAGGCAAATGAG |
Internal bar code: | TCGTCGCGTCCTATAGTGAGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 646 |
LEAP-Seq percent confirming: | 70.7958 |
LEAP-Seq n confirming: | 2829 |
LEAP-Seq n nonconfirming: | 1167 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTCCACGTGACAGCAACTC |
Suggested primer 2: | GGGCTTGAGGCATAGTTACG |