| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.196264 |
| Chromosome: | chromosome 3 |
| Location: | 8153884 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200550 | HYD3 | Iron hydrogenase/prelamin A binding protein; (1 of 1) PTHR11615:SF103 - CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NARFL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAAACAGCTGTGGCCGTACGCCCGTACC |
| Internal bar code: | AACGGATGGGCCCACTATCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 99.5675 |
| LEAP-Seq n confirming: | 8749 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACGGATAATGCACGTTG |
| Suggested primer 2: | AGGCCCCAATTTTCAATACC |