| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.196286 |
| Chromosome: | chromosome 4 |
| Location: | 1187073 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215600 | (1 of 1) PTHR32183:SF6 - THIOCYANATE METHYLTRANSFERASE 1-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGAGCCTCAGTAGCTGCGCAGCTCAGGC |
| Internal bar code: | AAGCGTGTCCGGGACCTTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 965 |
| LEAP-Seq percent confirming: | 96.1448 |
| LEAP-Seq n confirming: | 1222 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCGAATTCCTTAGATTG |
| Suggested primer 2: | GCGACTTCTTCAAGTTTGCC |