| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196287 |
| Chromosome: | chromosome_10 |
| Location: | 3151628 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g441900 | FAP145 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCTACGAACGCGGCGAAAAAGACTTGTCC |
| Internal bar code: | GCCGACTGTCGCCTCCTCTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 516 |
| LEAP-Seq percent confirming: | 99.4608 |
| LEAP-Seq n confirming: | 5165 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGTACGGCCCTGGACTA |
| Suggested primer 2: | CTTGTACAGCTTGCTCGCAG |